Reverse transcription-loop-mediated isothermal amplification for the detection of rodent coronaviruses

► A RT-LAMP assay detecting rodent coronaviruses was developed. ► The detection sensitivity was 3.2-fold higher than that of RT-PCR. ► The detection sensitivity was 31.6-fold lower than that of nested RT-PCR. ► In a practical diagnostic test, the detection sensitivity of duplicate RT-LAMP with regard to the nested RT-PCR was 87.5%. ► In a practical diagnostic test, the detection specificity of duplicate RT-LAMP with regard to the nested RT-PCR was 100%.