Development of an antigen-capture ELISA for the detection of avian leukosis virus p27 antigen

► The limit of detection of the AC-ELISA was 101.79 TCID50 units of HLJ09MDJ-1. ► The AC-ELISA had no cross-reaction with other viruses that infect chickens. ► The AC-ELISA can detect ALV in cloacal swabs as early as 12 d.p.i. ► The result of the AC-ELISA detected field samples approached to that of PCR test.