Low abundance drug resistance variants in transmitted HIV drug resistance surveillance specimens identified using tagged pooled pyrosequencing

► A novel tagged pooled pyrosequencing (TPP) platform for HIV drug resistance (DR) testing. ► TPP produces HIV DR profiles concordant with conventional bulk sequencing. ► TPP quantitatively identifies low abundance DR variants (LADRV) with high accuracy. ► TPP is cost-effective for HIV DR surveillance test with added benefit of LADRV detection.