Fluorescence polarization-based assay using N-glycan-conjugated quantum dots for screening in hemagglutinin blockers for influenza A viruses

► Fluorescent nanoparticles displaying multiple N-glycan with α-sialosides were constructed. ► Fluorescence polarization was detected in interaction of the particle with the HA. ► Competitive inhibition with a derivative of sialic acid was observed between the α-sialosides and the HA. ► The FP assay is useful for screening of HA blockers.