Development of a reverse-transcription loop-mediated isothermal amplification method for detection of rabbit hemorrhagic disease virus

► A novel method of one-step RT-LAMP detection of RHDV was described. ► Four specific primers were designed according to 27 isolates of RHDV-VP60. ► Specific amplification reaction for RHDV-VP60 different from other caliciviruses. ► A rapid, simple, sensitive and visually diagnostic method on RHDV was constructed.