Comparative analysis of different methods to enhance porcine circovirus 2 replication

► We compared the effects of different methods on PCV2 replication in vitro. ► IL-2, ConA and d-glucosamine increased PCV2 yield more effectively. ► IL-2, ConA, d-glucosamine and MβCD consistently increased PCV2 infection. ► Combination of ConA, MβCD and d-glucosamine increased PCV2 yield significantly.