In situ hybridisation assay for localisation of rabbit calicivirus Australia-1 (RCV-A1) in European rabbit (Oryctolagus cuniculus) tissues

► In situ hybridisation (ISH) protocol was developed for RCV-A1. ► Positive ISH signal limited to single epithelial duodenum cells. ► Viral RNA was detected in several other tissues using RT-PCR. ► No histo-pathological changes was detected in the infected tissues.