Evaluation of two (semi-)nested VP1 based-PCRs for typing enteroviruses directly from cerebral spinal fluid samples

► The EV-A and -B protocol was more sensitive than the Codehop protocol. ► The detection limit of the two protocols varied between the types. ► The Codehop protocol showed a high degree of aspecific amplification. ► Fifteen/twenty-two CSFs could be typed; E30 (9), CAV9 (2), E6, E11, E21, E25.