Electronic microarray assays for avian influenza and Newcastle disease virus

► 3 PCR-based microarray assays were developed for detection and typing of AIV and NDV. ► Assay 1 detects AIV/NDV and pathotypes NDV. Assays 2 and 3 identify AIV H- and N-types. ► Mixed infections were detected by the multiplexed assay and the AIV H-typing assay.