Rapid and sensitive detection of shrimp yellow head virus using loop-mediated isothermal amplification and a colorogenic nanogold hybridization probe

► Two techniques were combined into a more efficient and affordable protocol for YHV detection. ► The method consists of LAMP and hybridization with an AuNP-probe followed by salt induction of probe-particle aggregation. ► Detection sensitivity of YHV LAMP/AuNP method was comparable to that of nested RT-PCR.