Development of murine monoclonal antibodies with potent neutralization effects on enterovirus 71

► Recombinant virus-like particles were used as the immunogens to successfully develop monoclonal antibodies. ► Murine monoclonal antibodies which potently neutralized enterovirus 71 (EV71) were produced. ► The anti-EV71 neutralizing monoclonal antibodies targeted the VP1 subunit protein.