Antigen-capture ELISA for the detection of Rift Valley fever virus nucleoprotein using new monoclonal antibodies

► An RVFV NP-detection ELISA system using novel MAbs was developed. ► Epitope mapping showed that MAbs recognized highly conserved epitopes on RVFV NP. ► The ELSIA system is useful for diagnosis of RVFV infection in humans and animals.