Rapid and sensitive detection of mud crab Scylla serrata reovirus by a reverse transcription loop-mediated isothermal amplification assay

► Scylla serrata reovirus (SsRV) is one of the most prevalent viral pathogens of the mud crab (S. serrata). ► A one-step, single-tube RT-LAMP assay for the rapid detection of SsRV by simple visual inspection of amplification products was developed. ► The SsRV RT-LAMP assay would be very useful in simply equipped laboratories in developing countries.