Detection of enterovirus 71 using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

► The reverse loop-mediated isothermal amplification (RT-LAMP) method for EV71 was established. ► Compared with conventional RT-PCR, the EV71 RT-LAMP performed well in the aspect of detection time, sensitivity, specificity and visibility. ► RT-LAMP assay for EV71 could be used as a clinical diagnostic method in laboratories with limited equipment, especially under field conditions.