Detection of lamivudine- or adefovir-resistant hepatitis B virus mutations by a liquid array

► The multi-analyte suspension array based on liquid-phase hybridization can detect 96 samples and 96 mutations simutaneously. ► The microsphere used in the methods can be combined with more probes than a membrane or solid chip so that it needs shorter hybirdization time and has more specificity and sensitivity. ► The whole detection procedure was accomplised within 8 h and it is a simple and high-throughput method for resistance detection in many clinical samples. ► It is a cost-effective method for resistance surveillance.