Multiplex high resolution melting assay for estimation of Porcine Endogenous Retrovirus (PERV) relative gene dosage in pigs and detection of PERV infection in xenograft recipients

► High resolution melting chemistry accurately estimates levels of PERV provirus. ► Utility as multiplex PCR to test PERV infection in xenograft recipients whilst checking for microchimerism and sample quality. ► Auckland Island animals with low PERV gene dosage were identified for future selective breeding. ► No PERV infection found in human recipients of porcine pancreatic islets.