Polyclonal antibodies against properly folded Dengue virus NS1 protein expressed in E. coli enable sensitive and early dengue diagnosis

► Properly folded NS1 protein expressed in E. coli is suitable for DENV diagnosis. ► Oligomerization of NS1 is important to its immunogenicity. ► Anti-NS1 polyclonal antibodies are able to detect NS1 in DENV-positive human serum.