Expression of the influenza M2 protein in three different eukaryotic cell lines

► The M2 protein of the influenza A virus is a candidate for a novel vaccine. ► The expression of the cloned M2 gene in three eukaryotic cell lines is described. ► Expression efficiency was demonstrated by IF staining, ELISA and Western blot. ► The scale of expression is important for evaluating its potential in vaccine studies. ► Differences of M2 expression levels were observed depending on the cell line.