A multiple SYBR Green I-based real-time PCR system for the simultaneous detection of porcine circovirus type 2, porcine parvovirus, pseudorabies virus and Torque teno sus virus 1 and 2 in pigs

► SYBR Green multiple real-time PCR system for detection of five DNA porcine viruses distinguishes between all the five viral agents and proved to be specific. ► Coefficients of variation were low for both intra-assay and inter-assay variability. ► Useful tool for epidemiological studies and disease management.