Usefulness of the rapid determination system of viral genome sequences in human stool specimens

► The rapid determination system of viral genome sequences (RDV) was applied for the detection of viral genomes in human stools. ► The effect of nuclease treatment of the RDV method was examined. ► The RDV method has potential for the detection of viral genomes in stool specimens. ► The procedure without a step of nuclease treatment appears to be sensitive.