Tissue blot immunoassay and direct RT-PCR of cucumoviruses and potyviruses from the same NitroPure nitrocellulose membrane

▶ Viral RNA retrieved by RT-PCR from nitrocellulose membranes following immunoassay. ▶ Tissue blot immunoassay improved by Triton X-100 to remove green plant sap color. ▶ Nitrocellulose membranes are effective for archiving plant RNA viruses. ▶ Nitropure nitrocellulose compares to FTA Cards for RT-PCR recovery of plant viral RNA. ▶ Direct sequencing of amplicons for virus species/strain identification.