کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6135406 | 1223621 | 2010 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and inter-laboratory evaluation of real-time PCR assays for the detection of pospiviroids
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development and inter-laboratory evaluation of real-time PCR assays for the detection of pospiviroids Development and inter-laboratory evaluation of real-time PCR assays for the detection of pospiviroids](/preview/png/6135406.png)
چکیده انگلیسی
Assays based on real-time PCR (TaqMan®) that can detect a number of viroids in the genus Pospiviroid have been developed and evaluated. The assays are designed for detecting viroids from tomato leaf material but detection from other solanaceous hosts of these viroids has been confirmed. These methods have been validated by nine laboratories and comprise a reliable set of assays for the detection of CEVd, TASVd, CLVd and a generic assay which will detect the six viroids of concern to European tomato growers: PSTVd, TCDVd, CEVd, CLVd, TASVd and CSVd.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 169, Issue 1, October 2010, Pages 207-210
Journal: Journal of Virological Methods - Volume 169, Issue 1, October 2010, Pages 207-210
نویسندگان
Wendy Monger, Jenny Tomlinson, Neil Booonham, Mojca Virscek Marn, Irena Mavric Plesko, Valérie Molinero-Demilly, Xavier Tassus, Ellis Meekes, Marcel Toonen, Lambros Papayiannis, Zoila Perez-Egusquiza, Natasa Mehle, Claudia Jansen, Steen Lykke Nielsen,