کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6135433 | 1223650 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and applications of a monoclonal antibody to a recombinant beak and feather disease virus (BFDV) capsid protein
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development and applications of a monoclonal antibody to a recombinant beak and feather disease virus (BFDV) capsid protein Development and applications of a monoclonal antibody to a recombinant beak and feather disease virus (BFDV) capsid protein](/preview/png/6135433.png)
چکیده انگلیسی
The development of diagnostic assays for detecting beak and feather disease virus (BFDV) has traditionally been hampered by the difficulty associated with producing suitable reagents, namely purified virus and polyclonal antibodies. In an effort to develop a consistent and standardised source of antibody, a monoclonal antibody to a recombinant BFDV capsid protein has been developed and its use in western blotting, immunohistochemistry (IHC), ELISA and haemagglutination-inhibition (HI) assays characterised. The antibody was specific for both the recombinant BFDV capsid protein and the whole virus and had similar optimal titres when used in western blotting and IHC. The antibody also had HI activity and detected BFDV virus from three genera of psittacine birds, including the recently described cockatiel BFDV isolate. The monoclonal antibody should have widespread application in both research and the development of diagnostic assays for BFDV.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 147, Issue 2, February 2008, Pages 206-212
Journal: Journal of Virological Methods - Volume 147, Issue 2, February 2008, Pages 206-212
نویسندگان
Patrick L. Shearer, Nicolai Bonne, Phillip Clark, Margaret Sharp, Shane R. Raidal,