کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6135443 1223650 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Real-time RT-PCR assay for rapid and specific detection of classical swine fever virus: Comparison of SYBR Green and TaqMan MGB detection methods using novel MGB probes
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Real-time RT-PCR assay for rapid and specific detection of classical swine fever virus: Comparison of SYBR Green and TaqMan MGB detection methods using novel MGB probes
چکیده انگلیسی

Real-time PCR is an accurate, rapid and reliable method that can be used for the detection and also for the quantitation of specific DNA molecules. The basic principle is the recurring measurement of a fluorescent signal, which is proportional to the amount of amplification product. In our trial two detection systems were tested for classical swine fever virus (CSFV) detection and for its discrimination from other pestiviruses; non-specific dsDNA-binding dye SYBR Green and specific fluorogenic TaqMan MGB probes. Real-time RT-PCR assays were evaluated for diagnostic sensitivity and specificity by different pestiviral reference and field strains. With both approaches, SYBR Green and TaqMan probes, respectively, all of the CSFV strains isolated on cell culture were detected and also clearly distinguished from other pestiviruses. However, the established one-step real-time TaqMan RT-PCR assay was shown to be more appropriate for pestivirus quantitation, it reduces the risk of contamination and is less time consuming.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 147, Issue 2, February 2008, Pages 257-264
نویسندگان
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