Mixed human intra- and inter-subtype infections with the parasite Blastocystis sp.

► Current subtyping methods underestimate mixed human infections with Blastocystis sp. ► First evidence of mixed intra- and inter-subtype infections with Blastocystis in a same individual ► Molecular identification of several isolates belonging to three subtypes in a same patient ► Large intra-subtype 3 diversity and genetic homogeneity of subtype 4 isolates ► Identification of patients at high risk of mixed infection in further epidemiological studies