کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6137713 | 1594022 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synthetic peptides as an antigenic base in an ELISA for laboratory diagnosis of schistosomiasis mansoni
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
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چکیده انگلیسی
A pool of five synthetic peptides was used as an antigenic base in an ELISA (ELISA-Pp) for laboratory diagnosis of Schistosoma mansoni. Serum samples were obtained from individuals with acute (n = 23) and chronic (n = 30) schistosomiasis, with other parasitoses (n = 39) or without parasitic infections (n = 100). ELISA-Pp was compared with other immunoenzymatic methods for detection of IgM (IgM-ELISA) or IgG (IgG-ELISA) as well as an immunofluorescence test for detection of IgM antibodies (IgM-IFT). The sensitivity and specificity of ELISA-Pp was 86.8% and 94.2% when tested on the schistosomiasis group and the non-schistosomiasis group, respectively. Comparison of ELISA-Pp with other serological methods resulted in κ concordance indices varying from 0.59 to 0.75. Evaluation of anti-peptide IgG antibodies showed higher levels in patients with acute compared with chronic schistosomiasis (P = 0.001). ELISA-Pp showed satisfactory sensitivity and high specificity and may constitute a potentially useful method for laboratory diagnosis of schistosomiasis mansoni.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Transactions of the Royal Society of Tropical Medicine and Hygiene - Volume 102, Issue 4, April 2008, Pages 360-366
Journal: Transactions of the Royal Society of Tropical Medicine and Hygiene - Volume 102, Issue 4, April 2008, Pages 360-366
نویسندگان
Edward José de Oliveira, HermÃnia Yohko Kanamura, Kioko Takei, Rosario Dominguez Crespo Hirata, Luiz Carlos Pedrosa Valli, Nga Yen Nguyen, Izabel de Carvalho Rodrigues, Amélia Ribeiro de Jesus, Mário Hiroyuki Hirata,