کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6138667 1594220 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enhancing dengue virus maturation using a stable furin over-expressing cell line
ترجمه فارسی عنوان
افزایش بلوغ ویروس دنگ با استفاده از یک سلول پایدار فورین بیش از حد بیان
کلمات کلیدی
فلوویروس، ویروس دنگی ویروس نیل غربی، بلوغ ویروسی، خنثی سازی آنتی بیوتیک، فورین،
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
چکیده انگلیسی


- Construction of a Vero cell line that expresses high levels of human furin.
- Over-expression of furin increases maturation efficiency of infectious flaviviruses.
- Homogeneously mature DENV populations may simplify structural studies.

Flaviviruses are positive-stranded RNA viruses that incorporate envelope (E) and premembrane (prM) proteins into the virion. Furin-mediated cleavage of prM defines a required maturation step in the flavivirus lifecycle. Inefficient prM cleavage results in structurally heterogeneous virions with unique antigenic and functional characteristics. Recent studies with dengue virus suggest that viruses produced in tissue culture cells are less mature than those produced in primary cells. In this study, we describe a Vero cell line that ectopically expresses high levels of human furin (Vero-furin) for use in the production of more homogenous mature flavivirus populations. Laboratory-adapted and clinical dengue virus isolates grow efficiently in Vero-furin cells. Biochemical and structural techniques demonstrate efficient prM cleavage in Vero-furin derived virus preparations. These virions also were less sensitive to neutralization by antibodies that bind efficiently to immature virions. This furin-expressing cell line will be of considerable utility for flavivirus neutralization and structural studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 497, October 2016, Pages 33-40
نویسندگان
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