کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6138677 | 1594227 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An in vitro reprogrammable antiviral RISC with size-preferential ribonuclease activity
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
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چکیده انگلیسی
Infection of Nicotiana benthamiana plants with Tomato bushy stunt virus (TBSV) mutants compromised for silencing suppression induces formation of an antiviral RISC (vRISC) that can be isolated using chromatography procedures. The isolated vRISC sequence-specifically degrades TBSV RNA in vitro, its activity can be down-regulated by removing siRNAs, and re-stimulated by exogenous supply of siRNAs. vRISC is most effective at hydrolyzing the ~4.8Â kb genomic RNA, but less so for a ~2.2Â kb TBSV subgenomic mRNA (sgRNA1), while the 3â² co-terminal sgRNA2 of ~0.9Â kb appears insensitive to vRISC cleavage. Moreover, experiments with in vitro generated 5â² co-terminal viral transcripts show that RNAs of ~2.7Â kb are efficiently cleaved while those of ~1.1Â kb or shorter are unaffected. The isolated antiviral ribonuclease complex fails to degrade ~0.4Â kb defective interfering RNAs (DIs) in vitro, agreeing with findings that in plants DIs are not targeted by silencing.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 490, March 2016, Pages 41-48
Journal: Virology - Volume 490, March 2016, Pages 41-48
نویسندگان
Rustem T. Omarov, Jessica Ciomperlik, Herman B. Scholthof,