کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6138862 1594229 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Two mutations in the vif gene of maedi-visna virus have different phenotypes, indicating more than one function of Vif
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Two mutations in the vif gene of maedi-visna virus have different phenotypes, indicating more than one function of Vif
چکیده انگلیسی


- The role of MVV Vif in neutralizing ovine APOBEC3Z2-Z3 is confirmed.
- A W98R mutation in Vif disrupts Vif-APOBEC3Z2-Z3 interaction.
- A P205S mutation in Vif together with a mutation in CA impairs virus replication.
- The phenotype of the CA(L120R)-Vif(P205S) double mutant is cell-type specific.
- No hypermutation is associated with the CA(L120R)-Vif(P205S) phenotype.

Like most other lentiviruses, maedi-visna virus (MVV) requires Vif for replication in natural target cells and in vivo. Here, we show that Vif-deficient MVV accumulates G-A mutations in the sequence context characteristic of ovine APOBEC3, consistent with a role of MVV Vif in neutralizing APOBEC3. We studied two point mutations in the vif gene of MVV. One was a tryptophan to arginine mutation that affects the interaction with APOBEC3 and caused G-A hypermutation. The other mutation was a proline to serine mutation that together with a mutation in the capsid protein caused attenuated replication in fetal ovine synovial (FOS) cells but not in sheep choroid plexus (SCP) cells. There was no hypermutation associated with this mutation. These results suggest that MVV Vif exerts more than one function and that there may be interaction between Vif and the capsid. The results also suggest the involvement of an unknown host factor in MVV Vif function.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 488, 15 January 2016, Pages 37-42
نویسندگان
, , , , , ,