کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6138909 | 1594229 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The dengue virus non-structural protein 1 (NS1) is secreted efficiently from infected mosquito cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Dengue virus NS1 is a glycoprotein of 46-50Â kDa which associates as a dimer to internal and cytoplasmic membranes and is also secreted, as a hexamer, to the extracellular milieu. However, the notion exist that NS1 is secreted only from infected vertebrate and not mosquito cells. In this work, evidence is presented showing that NS1 is secreted efficiently by infected mosquito cells. NS1 was detected in cell supernatants starting at 6Â hpi with a continuous concentration increase up to 24Â hpi. Nevertheless, cell viability showed an average cell survival of 97%. At variance with observations with vertebrate cells, NS1 does not seems to associate with the cytoplasmic membrane of insect cells. Finally, evidence is presented indicating that NS1 is secreted from insect cells as a barrel-shaped hexamer. These findings provide new insights into the biology of NS1 and open questions about the role of secreted NS1 in the vector mosquito.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 488, 15 January 2016, Pages 278-287
Journal: Virology - Volume 488, 15 January 2016, Pages 278-287
نویسندگان
Ana C. Alcalá, Fernando Medina, Arturo González-Robles, Lizbeth Salazar-Villatoro, Rogelio J. Fragoso-Soriano, Carlos Vásquez, Margot Cervantes-Salazar, Rosa M. del Angel, Juan E. Ludert,