کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6139081 | 1594233 | 2015 | 17 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Morphogenesis of respiratory syncytial virus in human primary nasal ciliated epithelial cells occurs at surface membrane microdomains that are distinct from cilia Morphogenesis of respiratory syncytial virus in human primary nasal ciliated epithelial cells occurs at surface membrane microdomains that are distinct from cilia](/preview/png/6139081.png)
- Respiratory syncytial virus (RSV) infects nasal ciliated epithelial cells.
- Virus morphogenesis occurs within filamentous projections distinct from cilia.
- The RSV N protein was not detected in the cilia at any time during infection.
- Trafficking of the F protein into the cilia occurred early in infection.
- Presence of the F protein in cilia correlated with impaired cilia function.
The distribution of cilia and the respiratory syncytial virus (RSV) nucleocapsid (N) protein, fusion (F) protein, attachment (G) protein, and M2-1 protein in human ciliated nasal epithelial cells was examined at between 1 and 5 days post-infection (dpi). All virus structural proteins were localized at cell surface projections that were distinct from cilia. The F protein was also trafficked into the cilia, and while its presence increased as the infection proceeded, the N protein was not detected in the cilia at any time of infection. The presence of the F protein in the cilia correlated with cellular changes in the cilia and reduced cilia function. At 5Â dpi extensive cilia loss and further reduced cilia function was noted. These data suggested that although RSV morphogenesis occurs at non-cilia locations on ciliated nasal epithelial cells, RSV infection induces changes in the cilia body that leads to extensive cilia loss.
Journal: Virology - Volume 484, October 2015, Pages 395-411