کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6139325 | 1594234 | 2015 | 9 صفحه PDF | دانلود رایگان |
- The complete genome of alfalfa dwarf virus is obtained.
- An integrated localization and interaction map for ADV is determined.
- ADV has a genome sequence similarity and evolutionary links with cytorhabdoviruses.
- ADV protein localization and interaction data show an association with the nucleus.
- ADV combines properties of both cytoplasmic and nuclear plant rhabdoviruses.
SummaryWe have determined the full-length 14,491-nucleotide genome sequence of a new plant rhabdovirus, alfalfa dwarf virus (ADV). Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3â²-N-P-P3-M-G-P6-L-5â². The ORFs are separated by conserved intergenic regions and the genome coding region is flanked by complementary 3â² leader and 5â² trailer sequences. Phylogenetic analysis of the nucleoprotein amino acid sequence indicated that this alfalfa-infecting rhabdovirus is related to viruses in the genus Cytorhabdovirus. When transiently expressed as GFP fusions in Nicotiana benthamiana leaves, most ADV proteins accumulated in the cell periphery, but unexpectedly P protein was localized exclusively in the nucleus. ADV P protein was shown to have a homotypic, and heterotypic nuclear interactions with N, P3 and M proteins by bimolecular fluorescence complementation. ADV appears unique in that it combines properties of both cytoplasmic and nuclear plant rhabdoviruses.
Journal: Virology - Volume 483, September 2015, Pages 275-283