کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6139349 | 1594231 | 2015 | 10 صفحه PDF | دانلود رایگان |

- The C-terminal domain of TuYV-RT is required for long-distance movement.
- CIPK7 from Arabidopsis interacts with RTCter in yeast and in plants.
- CIPK7 overexpression increases virus titer locally but not virus systemic movement.
- CIPK7 localizes to plasmodesmata.
- CIPK7 could be a defense protein regulating virus export.
Turnip yellows virus (TuYV), a phloem-limited virus, encodes a 74Â kDa protein known as the readthrough protein (RT) involved in virus movement. We show here that a TuYV mutant deleted of the C-terminal part of the RT protein (TuYV-âRTCter) was affected in long-distance trafficking in a host-specific manner. By using the C-terminal domain of the RT protein as a bait in a yeast two-hybrid screen of a phloem cDNA library from Arabidopsis thaliana we identified the calcineurin B-like protein-interacting protein kinase-7 (AtCIPK7). Transient expression of a GFP:CIPK7 fusion protein in virus-inoculated Nicotiana benthamiana leaves led to local increase of wild-type TuYV accumulation, but not that of TuYV-âRTCter. Surprisingly, elevated virus titer in inoculated leaves did not result in higher TuYV accumulation in systemic leaves, which indicates that virus long-distance movement was not affected. Since GFP:CIPK7 was localized in or near plasmodesmata, CIPK7 could negatively regulate TuYV export from infected cells.
Journal: Virology - Volume 486, December 2015, Pages 44-53