کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6139631 1594240 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation and characterization of a novel arenavirus harbored by Rodents and Shrews in Zhejiang province, China
ترجمه فارسی عنوان
جداسازی و مشخص کردن یک ویروس آرنویو جدید که توسط گاو و نوشیدنی در استان ژجیانگ چین انجام شده است
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
چکیده انگلیسی


- A novel arenavirus (Wenzhou virus) was identified in Zhejiang province, China.
- The virus is highly circulating in five species of rats and one species of shrews
- More efforts are needed to infer whether it is pathogenic to humans or not.

To determine the biodiversity of arenaviruses in China, we captured and screened rodents and shrews in Wenzhou city, Zhejiang province, a locality where hemorrhagic fever diseases are endemic in humans. Accordingly, arenaviruses were detected in 42 of 351 rodents from eight species, and in 12 of 272 Asian house shrews (Suncus murinus), by RT-PCR targeting the L segment. From these, a single arenavirus was successfully isolated in cell culture. The virion particles exhibited a typical arenavirus morphology under transmission electron microscopy. Comparison of the S and L segment sequences revealed high levels of nucleotide (>32.2% and >39.6%) and amino acid (>28.8% and >43.8%) sequence differences from known arenaviruses, suggesting that it represents a novel arenavirus, which we designated Wenzhou virus (WENV). Phylogenetic analysis revealed that all WENV strains harbored by both rodents and Asian house shrews formed a distinct lineage most closely related to Old World arenaviruses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 476, February 2015, Pages 37-42
نویسندگان
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