کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6140474 1594256 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cascade regulation of vaccinia virus gene expression is modulated by multistage promoters
ترجمه فارسی عنوان
مقررات آبرسانی بیان ژن ویروس واکسیناسیون توسط مولکول های چند مرحله ای مدولاسیون می شود
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
چکیده انگلیسی
Vaccinia virus contains ~200 genes classified temporally as early, intermediate or late. We analyzed 53 intermediate promoters to determine whether any have dual late promoter activity. Our strategy involved (i) construction of a cell line that stably expressed the three late transcription factors, (ii) infection with a vaccinia virus mutant that expresses RNA polymerase but neither intermediate nor late transcription factors, and (iii) transfection with plasmids containing a luciferase reporter regulated by an intermediate promoter. After confirming the specificity of the system for late promoters, we found that many intermediate promoters had late promoter activity, the strength of which correlated with a TAAAT at the initiator site and T-content from positions −12 to −8 of the coding strand. In contrast, intermediate promoter activity correlated with the A-content from positions −22 to −14. The sequence correlations were confirmed by altering the specificities of strict intermediate and late promoters.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 447, Issues 1–2, December 2013, Pages 213-220
نویسندگان
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