Characterizing monkeypox virus specific CD8+ T cell epitopes in rhesus macaques

- Peptides from MPXV proteins were screened for CD8+ T cell epitopes in rhesus.
- Two peptides from F8L/E9L protein were identified to trigger CD8+ T cell response.
- Peptide ISPDGCYSL is restricted by Mamu-A1⁎001.
- Peptide LTFDYVVTF is restricted by Mamu- A1⁎002.

To characterize T cell epitopes in monkeypox virus (MPXV) infected rhesus macaques, we utilized IFNγ Elispot assay to screen 400 predicted peptides from 20 MPXV proteins. Two peptides from the F8L protein, an analog of E9L protein in vaccinia, were found to elicit CD8+ T cell responses. Prediction and in vitro MHC binding analyses suggest that one is restricted by Mamu-A1⁎001 and another by Mamu-A1⁎002. The Mamu-A1⁎002 epitope is completely identical in all reported sequences for variola, vaccinia, cowpox and MPXV. The Mamu-A1⁎001 epitope is conserved in MPXV and vaccinia, and has one residue substitution (V6>I) in some cowpox sequences and all variola sequences. Given CD8+ T-cell epitopes from E9L were also identified in humans and mice, our data suggested that F8L/E9L may be a dominant pox viral protein for CD8+ T cell responses, and may be considered as a target when designing vaccines that target pox-specific T cell responses.