کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6140518 1594256 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mitigation of variation observed in a peripheral blood mononuclear cell (PBMC) based HIV-1 neutralization assay by donor cell pooling
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Mitigation of variation observed in a peripheral blood mononuclear cell (PBMC) based HIV-1 neutralization assay by donor cell pooling
چکیده انگلیسی


- PBMC neutralization assay is a valuable in vitro model for HIV vaccine evaluation.
- However, neutralizing titers spanned up to 4 logs when using different donor PBMC.
- Assay variability was antibody specific; greatest variation with polyclonal plasma.
- Pooling of multiple PBMC donors significantly reduced median inter-assay variation.
- PBMC pooling may allow for a more sustainable and standardized assay method.

Cultured primary peripheral blood mononuclear cells (PBMC) represent a potentially physiologic in vitro model of HIV-1 infection, but assessment of antibody-mediated HIV-1 neutralization using PBMC has been hindered by donor variability and lack of a sustainable individual PBMC source. To advance this model for HIV vaccine evaluation, intra- and inter-assay variability were assessed using monoclonal and polyclonal antibodies and PBMC targets from multiple HIV-seronegative donors. Inter-assay variability was introduced by using different PBMC for virus propagation, and more substantially, for assay targets. Neutralization titers varied by as much as 4 logs when using different individual donor PBMC as targets; variability was antibody-specific, with the greatest variation observed using an individual polyclonal plasma. Pooling of multiple PBMC donors significantly reduced median inter-assay variation to the level of intra-assay variation, suggesting a pathway forward for establishing a uniform, sustainable and standardized approach to the assessment of antibody function using a PBMC model.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 447, Issues 1–2, December 2013, Pages 240-248
نویسندگان
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