کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6140522 | 1594256 | 2013 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Engineered alphavirus replicon vaccines based on known attenuated viral mutants show limited effects on immunogenicity
ترجمه فارسی عنوان
واکسن های تکثیر شده با الف ویروس مهندسی شده مبتنی بر جهش های شناخته شده ویروسی ضعیف اثرات محدودی بر ایمنی زایی دارد
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
چکیده انگلیسی
The immunogenicity of alphavirus replicon vaccines is determined by many factors including the level of antigen expression and induction of innate immune responses. Characterized attenuated alphavirus mutants contain changes to the genomic 5â² UTR and mutations that result in altered non-structural protein cleavage timing leading to altered levels of antigen expression and interferon (IFN) induction. In an attempt to create more potent replicon vaccines, we engineered a panel of Venezuelan equine encephalitis-Sindbis virus chimeric replicons that contained these attenuating mutations. Modified replicons were ranked for antigen expression and IFN induction levels in cell culture and then evaluated in mice. The results of these studies showed that differences in antigen production and IFN induction in vitro did not correlate with large changes in immunogenicity in vivo. These findings indicate that the complex interactions between innate immune response and the repliconâ²s ability to express antigen complicate rational design of more potent alphavirus replicons.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 447, Issues 1â2, December 2013, Pages 254-264
Journal: Virology - Volume 447, Issues 1â2, December 2013, Pages 254-264
نویسندگان
Giulietta Maruggi, Christine A. Shaw, Gillis R. Otten, Peter W. Mason, Clayton W. Beard,