کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6140619 1594257 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An unbiased in vivo screen reveals multiple transcription factors that control HPV E6-regulated hTERT in keratinocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
An unbiased in vivo screen reveals multiple transcription factors that control HPV E6-regulated hTERT in keratinocytes
چکیده انگلیسی


- Yeast LacO-LacI system was adapted to identify promoter bound proteins in human cells.
- New transcriptional repressors were identified on the hTERT promoter with this system.
- These transcriptional repressors were regulated during E6-induced hTERT expression.

Activation of telomerase by human papillomavirus 16 (HPV16) E6 is a critical step for cell immortalization and transformation in human foreskin keratinocytes (HFKs). Multiple transcription factors have been identified as being involved in E6-induced hTERT expression. Here, we adapted an unbiased in vivo screen using a LacO-LacI system in human cells to discover hTERT promoter-interacting regulators. This approach allowed us to identify a novel hTERT repressor, Maz, which bound the hTERT promoter. E6 expression reduced Maz binding and correspondingly increased Sp1 binding at the hTERT promoter. Knockdown of Maz further increased histone acetylation, as well as hTERT expression in the presence of E6. Overall, these data indicate the utility of a novel screen for promoter-interacting and transcription-regulating proteins. These data also highlight multiple factors that normally regulate hTERT repression in HFKs, and therefore are targeted by E6 for hTERT expression.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 446, Issues 1–2, November 2013, Pages 17-24
نویسندگان
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