کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6140775 | 1594255 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced IRES activity by the 3â²UTR element determines the virulence of FMDV isolates
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Enhanced IRES activity by the 3â²UTR element determines the virulence of FMDV isolates Enhanced IRES activity by the 3â²UTR element determines the virulence of FMDV isolates](/preview/png/6140775.png)
چکیده انگلیسی
A reverse genetics approach was used to identify viral genetic determinants of the differential virulence displayed by two field foot-and-mouth disease virus (FMDV) strains (A/Arg/00 and A/Arg/01) isolated in Argentina during the 2000-2001 epidemics. A molecular clone of A/Arg/01 strain and viral chimeras containing the S-fragment or the internal ribosome entry site (IRES) of A/Arg/00 in the A/Arg/01 backbone were constructed and characterized. The IRES appeared as a determining factor of the lower level of A/Arg/00 replication in cell culture. High-throughput RNA probing revealed structural differences between both IRESs. Translation experiments using either synthetic viral RNAs (in vitro) or bicistronic plasmids (in vivo) showed that these IRESs' activities differ when the viral 3â² untranslated region (UTR) is present, suggesting that their function is differentially modulated by this region. This work provides experimental evidence supporting the role of the IRES-3â²UTR modulation in determining the level of FMDV replication in field strains.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 448, 5 January 2014, Pages 303-313
Journal: Virology - Volume 448, 5 January 2014, Pages 303-313
نویسندگان
Soledad GarcÃa-Nuñez, MarÃa Inés Gismondi, Guido König, AnalÃa Berinstein, Oscar Taboga, Elizabeth Rieder, Encarnación MartÃnez-Salas, Elisa Carrillo,