کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6140912 1594259 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Barrier to autointegration factor (BAF) inhibits vaccinia virus intermediate transcription in the absence of the viral B1 kinase
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Barrier to autointegration factor (BAF) inhibits vaccinia virus intermediate transcription in the absence of the viral B1 kinase
چکیده انگلیسی


- The role of the B1-BAF axis in the vaccinia lifecycle is poorly understood.
- Vaccinia B1 kinase is needed for viral intermediate transcription.
- Depletion of BAF strongly rescues the transcriptional defect caused by the loss of B1.
- Depletion of BAF has only a modest effect on non-vaccinia promoters.
- BAF can act as a transcriptional repressor, but is inactivated by the B1 kinase.

Barrier to autointegration factor (BAF/BANF1) is a cellular DNA-binding protein found in the nucleus and cytoplasm. Cytoplasmic BAF binds to foreign DNA and can act as a defense against vaccinia DNA replication. To evade BAF, vaccinia expresses the B1 kinase, which phosphorylates BAF and blocks its ability to bind DNA. Interestingly, B1 is also needed for viral intermediate gene expression via an unknown mechanism. Therefore, we evaluated the impact of B1-BAF signaling on vaccinia transcription. Strikingly, the decrease in vaccinia transcription caused by loss of B1 can be rescued by depletion of BAF. The repressive action of BAF is greatest on a viral promoter, and is more modest when non-vaccinia promoters are employed, which suggests BAF acts in a gene specific manner. These studies expand our understanding of the role of the B1 kinase during infection and provide the first evidence that BAF is a defense against viral gene expression.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 444, Issues 1–2, September 2013, Pages 363-373
نویسندگان
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