کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6140913 | 1594259 | 2013 | 10 صفحه PDF | دانلود رایگان |
- Twenty eight novel Bacillus pumilus bacteriophages isolated; nine sequenced; six annotated.
- Categorized into two types, BpA and BpB.
- High similarity despite diverse geographic and temporal isolation.
- First B. pumilus phage genome annotation.
Twenty-eight bacteriophages infecting the local host Bacillus pumilus BL-8 were isolated, purified, and characterized. Nine genomes were sequenced, of which six were annotated and are the first of this host submitted to the public record. The 28 phages were divided into two groups by sequence and morphological similarity, yielding 27 cluster BpA phages and 1 cluster BpB phage, which is a BL-8 prophage. Most of the BpA phages have a host range restricted to distantly related strains, B. pumilus and B. simplex, reflecting the complexities of Bacillus taxonomy. Despite isolation over wide geographic and temporal space, the six cluster BpA phages share most of their 23 functionally annotated protein features and show a high degree of sequence similarity, which is unique among phages of the Bacillus genera. This is the first report of B. pumilus phages since 1981.
Journal: Virology - Volume 444, Issues 1â2, September 2013, Pages 374-383