کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6141039 | 1227201 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of two functional nuclear localization signals in the capsid protein of duck circovirus
ترجمه فارسی عنوان
شناسایی دو سیگنال محلی سازی هسته کارکردی در پروتئین کپسید دایرو ویروس اردک
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
چکیده انگلیسی
The capsid protein (CP) of duck circovirus (DuCV) is the major immunogenic protein and has a high proportion of arginine residues concentrated at the N terminus of the protein, which inhibits efficient mRNA translation in prokaryotic expression systems. In this study, we investigated the subcellular distribution of DuCV CP expressed via recombinant baculoviruses in Sf9 cells and the DNA binding activities of the truncated recombinant DuCV CPs. The results showed that two independent bipartite nuclear localization signals (NLSs) situated at N-terminal 1-17 and 18-36 amino acid residue of the CP. Moreover, two expression level regulatory signals (ELRSs) and two DNA binding signals (DBSs) were also mapped to the N terminus of the protein and overlapped with the two NLSs. The ability of CP to bind DNA, coupled with the karyophilic nature of this protein, strongly suggests that it may be responsible for nuclear targeting of the viral genome.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 436, Issue 1, 5 February 2013, Pages 112-117
Journal: Virology - Volume 436, Issue 1, 5 February 2013, Pages 112-117
نویسندگان
Qi-Wang Xiang, Jin-Feng Zou, Xin Wang, Ya-Ni Sun, Ji-Ming Gao, Zhi-Jing Xie, Yu Wang, Yan-Li Zhu, Shi-Jin Jiang,