Biological characteristics and propagation of human rhinovirus-C in differentiated sinus epithelial cells

► A system was developed to grow differentiated sinus epithelial cells at air-liquid interface. ► These cells were used as a culture system for HRV-A and HRV-C viruses. ► Replication kinetics for HRV-C15 are similar to those of HRV-A16. ► Like other HRV types, HRV-C15 is neutralized by low pH (4.5). ► In contrast to most HRV-A and HRV-B, HRV-C types grew equally well at 34-37  °C.