کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6141090 | 1227202 | 2012 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sequential deletion of genes from the African swine fever virus genome using the cre/loxP recombination system
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A method has been established to sequentially delete combinations of genes from the ASFV genome to test the effect on virus replication and host responses to infection. Initially the ASFV genes MGF505 2R and MGF505 3R and a truncated MGF360 9L gene were deleted from the genome of the tissue-culture adapted ASFV strain BA71V and replaced with bacteriophage loxP sequences flanking the beta-glucuronidase (GUS) marker gene to create recombinant virus VÎMGF-GUS. Subsequently the GUS gene was removed by site-specific recombination between the two loxP sites involving expression of the bacteriophage Cre recombinase enzyme to create recombinant virus VÎMGFÎGUS. The EP402R and EP153R genes were subsequently deleted from the genome of VÎMGFÎGUS, using the same GUS marker gene, to construct virus VÎMGFÎCD2-Lectin-GUS. These sequential deletions of ASFV genes were shown not to alter virus replication significantly.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 433, Issue 1, 10 November 2012, Pages 142-148
Journal: Virology - Volume 433, Issue 1, 10 November 2012, Pages 142-148
نویسندگان
Charles C. Abrams, Linda K. Dixon,