Multiply-primed rolling circle amplification of human papillomavirus using sequence-specific primers

► Rolling circle amplification of HPV using specific primers, followed by Sanger seq. ► Comparing RCA efficiency when using HPV-specific primers against random hexamers. ► Increased RCA efficiency and reduced background amplification with HPV-primers. ► Obtaining high-quality sequence reads from RCA-products, even for low-input amount. ► Identification of several interesting polymorphic positions in 4 clinical samples.