Characterization of a recombinant canine coronavirus with a distinct receptor-binding (S1) domain

► Characterization of a typical canine alphacoronavirus, CCoV-A76. ► CCoV-A76 is serologically distinct from other characterized CCoVs. ► The virus replicates well in canine cells but not in feline cells. ► CCoV-A76 can use canine Aminopeptidase N (cAPN) but not feline APN (fAPN) for entry. ► The virus' spike is a recombinant form between serotype I and II CCoV spikes.