Alfalfa mosaic virus replicase proteins, P1 and P2, localize to the tonoplast in the presence of virus RNA

► AMV replication proteins assemble on multivesicular bodies (MVBs). ► P1 replicase protein expressed alone localizes to the MVBs. ► P2 replicase protein expressed alone accumulates throughout the cytoplasm. ► P1 recruits P2 to the MVBs. ► A replication competent RNA is required for the complex to localize on the vacuolar membrane, the tonoplast.