کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6141263 | 1227221 | 2012 | 11 صفحه PDF | دانلود رایگان |
RNA replication of dengue virus (DENV) requires an RNA-RNA mediated circularization of the viral genome, which includes at least three sets of complementary RNA sequences on both ends of the genome. The 5â² and the 3â² untranslated regions form several additional RNA elements that are involved in regulation of translation and required for RNA replication. Communication between the genomic termini results in a structural reorganization of the RNA elements, forming a functional RNA panhandle structure. Here we report that the sequence composition downstream of the 5â² CS element in the capsid gene, designated as downstream CS (dCS) sequence - but not the capsid protein - also influences the ability of the viral genome to circularize and hence replicate by modulating the topology of the 5â² end. These results provide insights for the design of reporter sub-genomic and genomic mosquito-borne flavivirus constructs and contribute to the understanding of viral RNA replication.
⺠Mosquito-borne flavivirus capsid-coding sequence composition affects RNA replication. ⺠Flavivirus capsid-coding sequence affects 5ⲠRNA topology and genome circularization. ⺠New insights for the design of mosquito-borne flavivirus reporter constructs.
Journal: Virology - Volume 422, Issue 2, 20 January 2012, Pages 346-356