کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6141372 | 1227244 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Base-pairing promotes leader selection to prime in vitro influenza genome transcription
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The requirements for alignment of capped leader sequences along the viral genome during influenza transcription initiation (cap-snatching) have long been an enigma. In this study, competition experiments using an in vitro transcription assay revealed that influenza virus transcriptase prefers leader sequences with base complementarity to the 3â²-ultimate residues of the viral template, 10 or 11 nt from the 5â² cap. Internal priming at the 3â²-penultimate residue, as well as prime-and-realign was observed. The nucleotide identity immediately 5â² of the base-pairing residues also affected cap donor usage. Application to the in vitro system of RNA molecules with increased base complementarity to the viral RNA template showed stronger reduction of globin RNA leader initiated influenza transcription compared to those with a single base-pairing possibility. Altogether the results indicated an optimal cap donor consensus sequence of 7mG-(N)7-8-(A/U/G)-(A/U)-AGC-3â².
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 409, Issue 1, 5 January 2011, Pages 17-26
Journal: Virology - Volume 409, Issue 1, 5 January 2011, Pages 17-26
نویسندگان
Christina Geerts-Dimitriadou, Mark P. Zwart, Rob Goldbach, Richard Kormelink,